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Fluorescence Lifetime Imaging Microscopy (FLIM) Solutions for Confocal Microscopes

Becker & Hickl modules can turn a broad range of confocal microscope into FLIM systems. Upgrade packages include electronics, detectors, lasers, scanners, computers, software and accessories. Solutions are available for many microscope manufactures including:

  • Zeiss
  • Leica
  • Nikon
  • Olympus
  • Sutter
  • Others – please enquire

FLIM upgrades feature:

  • Fluorescence lifetime imaging with picosecond resolution
  • Excitation by picosecond diode lasers
  • Confocal detection
  • Simultaneous detection in several wavelength intervals
  • Multi-dimensional TCSPC techniques
  • Excellent time resolution and sensitivity
  • Zoom and image rotation functions of microscope applicable
  • Work at any scan rate

Confocal Scanner – DCS-120

For those systems that may not already have confocal scanning capability, we have that covered for you. The DCS-120 be used to convert an existing conventional microscope into a fully functional confocal or multiphoton laser scanning microscope with TCSPC detection. Due to its fast beam scanning and its high sensitivity the DCS-120 system is compatible with live cell imaging. DCS-120 functions allow:

  • simultaneous recording of FLIM or steady-state fluorescence images simultaneously in two fully parallel wavelength channels,
  • laser wavelength multiplexing,
  • time-series FLIM, time series recording,
  • Z stack FLIM,
  • phosphorescence lifetime imaging (PLIM),
  • fluorescence lifetime-transient scanning (FLITS)
  • FCS recording.

Our team of application specialist will work with you to define the most appropriate modules to create your upgrade system. We can provide on-site installation and training at your option.

  • Compact TCSPC system, coupled to Laptop computer via Thunderbolt interface
  • One or two SPC-160pcie TCSPC modules
  • One DCC-100pcie detector controller
  • One GVD-120pcie scan controller
  • Photon distribution mode, Time-tag mode
  • Time channel width down to 813 fs
  • Electronic IRF 7 ps FWHM
  • High count rate
  • Unprecedented timing stability
  • Laptop computer with extension box
  • Detector Control Module included

  • Compact TCSPC system based on SPC-130
  • Standard fluorescence decay recording
  • On-line FCS recording
  • Photon distribution mode, Time-tag mode
  • Fast triggered sequential-recording
  • Time channel width down to 813 fs
  • Electronic IRF 7 ps FWHM
  • High count rate
  • Unprecedented timing stability
  • Laptop computer with extension box
  • Detector Control Module included

  • Compact TCSPC system based on SPC-150
  • Single detector FLIM
  • On-line FCS recording
  • Lifetime Imaging in histogram and time-tag modes
  • Multi-spectral FLIM (optional multi-wavelength detector)
  • Time channel width down to 813 fs
  • Unprecedented timing stability
  • 10 MHz total saturated count rate
  • Laptop computer with extension box
  • Detector Control Module included

  • Compact TCSPC system based on 2 x SPC-150
  • Two fully parallel TCSPC channels
  • Dual detector FLIM
  • On-line FCS recording
  • Lifetime Imaging in histogram and time-tag modes
  • Multi-spectral FLIM (optional multi-wavelength detector)
  • Time channel width down to 813 fs
  • Unprecedented timing stability
  • 20 MHz total saturated count rate
  • Laptop computer with extension box
  • Detector Control Module included

  • Compact TCSPC system based on 4 x SPC-150
  • Four parallel TCSPC channels
  • Diffuse optical tomography
  • Ultra-high speed parallel multispectral FLIM
  • Lifetime Imaging in histogram and time-tag modes
  • Multi-spectral FLIM (optional multi-wavelength detector)
  • Time channel width down to 813 fs
  • Unprecedented timing stability
  • 40 MHz total saturated count rate
  • Photon distribution mode, Time-tag mode
  • Laptop computer with extension box

  • Compact TCSPC system based on SPC-830
  • Single detector FLIM
  • On-line FCS recording
  • Lifetime Imaging in histogram and time-tag modes
  • Multi-spectral FLIM (optional multi-wavelength detector)
  • Time channel width down to 813 fs
  • Unprecedented timing stability
  • 10 MHz total saturated count rate
  • Laptop computer with extension box
  • Detector Control Module included

  • Confocal scanning principle
  • Fast scanning by galvanometer mirrors
  • Excitation by two ps diode lasers or tuneable ps laser
  • Detection in two spectral or polarisation channels
  • Recording by bh’s multidimensional TCSPC technique
  • High-throughput dual-channel parallel TCSPC architecture
  • High-efficiency GaAsP hybrid detectors
  • Phosphorescence (PLIM) function included
  • Compact electronics, controlled by laptop computer
  • Scan field up to 15 mm diameter
  • Pixel numbers from 16 x 16 to 2048 x 2048
  • Fast preview function
  • Interactive scanner control
  • Easy selection of scan field
  • Beam-stop function for single-point measurement
  • Single, double, or triple-exponential data analysis

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