Integrated TCSPC Systems

Fluorescence Lifetime Imaging Microscopy (FLIM) Solutions for Confocal Microscopes

Becker & Hickl modules can turn a broad range of confocal microscope into FLIM systems. Upgrade packages include electronics, detectors, lasers, scanners, computers, software and accessories. Solutions are available for many microscope manufactures including:

• Zeiss
• Leica
• Nikon
• Olympus
• Sutter
• Others – please enquire

FLIM upgrades feature:

• Fluorescence lifetime imaging with picosecond resolution
• Excitation by picosecond diode lasers
• Confocal detection
• Simultaneous detection in several wavelength intervals
• Multi-dimensional TCSPC techniques
• Excellent time resolution and sensitivity
• Zoom and image rotation functions of microscope applicable
• Work at any scan rate

Confocal Scanner – DCS-120

For those systems that may not already have confocal scanning capability, we have that covered for you. The DCS-120 be used to convert an existing conventional microscope into a fully functional confocal or multiphoton laser scanning microscope with TCSPC detection. Due to its fast beam scanning and its high sensitivity the DCS-120 system is compatible with live cell imaging. DCS-120 functions allow:

• simultaneous recording of FLIM or steady-state fluorescence images simultaneously in two fully parallel wavelength channels,
• laser wavelength multiplexing,
• time-series FLIM, time series recording,
• Z stack FLIM,
• phosphorescence lifetime imaging (PLIM),
• fluorescence lifetime-transient scanning (FLITS)
• FCS recording.

Our team of application specialist will work with you to define the most appropriate modules to create your upgrade system. We can provide on-site installation and training at your option.

• Compact TCSPC system, coupled to Laptop computer via Thunderbolt interface
• One or two SPC-160pcie TCSPC modules
• One DCC-100pcie detector controller
• One GVD-120pcie scan controller
• Photon distribution mode, Time-tag mode
• Time channel width down to 813 fs
• Electronic IRF 7 ps FWHM
• High count rate
• Unprecedented timing stability
• Laptop computer with extension box
• Detector Control Module included

• Compact TCSPC system based on SPC-130-EMN
• Standard fluorescence decay recording
• On-line FCS recording
• Photon distribution mode, Time-tag mode
• Fast triggered sequential-recording
• Time channel width down to 813 fs
• Electronic IRF 7 ps FWHM
• High count rate
• Unprecedented timing stability
• Laptop computer with extension box
• Detector Control Module included

• Compact TCSPC system based on SPC-150-N
• Single detector FLIM
• On-line FCS recording
• Lifetime Imaging in histogram and time-tag modes
• Multi-spectral FLIM (optional multi-wavelength detector)
• Time channel width down to 813 fs
• Unprecedented timing stability
• 10 MHz total saturated count rate
• Laptop computer with extension box
• Detector Control Module included

• Compact TCSPC system based on 2 x SPC-150-N
• Two fully parallel TCSPC channels
• Dual detector FLIM
• On-line FCS recording
• Lifetime Imaging in histogram and time-tag modes
• Multi-spectral FLIM (optional multi-wavelength detector)
• Time channel width down to 813 fs
• Unprecedented timing stability
• 20 MHz total saturated count rate
• Laptop computer with extension box
• Detector Control Module included

• Compact TCSPC system based on 4 x SPC-150-N
• Four parallel TCSPC channels
• Diffuse optical tomography
• Ultra-high speed parallel multispectral FLIM
• Lifetime Imaging in histogram and time-tag modes
• Multi-spectral FLIM (optional multi-wavelength detector)
• Time channel width down to 813 fs
• Unprecedented timing stability
• 40 MHz total saturated count rate
• Photon distribution mode, Time-tag mode
• Laptop computer with extension box

• Confocal scanning principle
• Fast scanning by galvanometer mirrors
• Excitation by two ps diode lasers or tuneable ps laser
• Detection in two spectral or polarisation channels
• Recording by bh’s multidimensional TCSPC technique
• High-throughput dual-channel parallel TCSPC architecture
• High-efficiency GaAsP hybrid detectors
• Phosphorescence (PLIM) function included
• Compact electronics, controlled by laptop computer
• Scan field up to 15 mm diameter
• Pixel numbers from 16 x 16 to 2048 x 2048
• Fast preview function
• Interactive scanner control
• Easy selection of scan field
• Beam-stop function for single-point measurement
• Single, double, or triple-exponential data analysis