What Is Time Correlated Single Photon Counting (TCSPC)Time-Correlated Single Photon Counting (TCSPC) is a technique to record low level light signals with picosecond time resolution. Typical applications are
TCSPC works best for
Time-Correlated Single Photon Counting is based on the detection of single photons of a periodical light signal, the measurement of the detection times of the individual photons and the reconstruction of the waveform from the individual time measurements. The method makes use of the fact that for low level, high repetition rate signals the light intensity is usually so low that the probability of detecting one photon in one signal period is much less than one. Therefore, the detection of several photons can be neglected and the principle shown in the figure below can be used: The detector signal consists of a train of randomly distributed pulses due to the detection of the individual photons. There are many signal periods without photons, other signal periods contain one photon pulse. Periods with more than one photons are very rare. When a photon is detected, the time of the corresponding detector pulse is measured. The events are collected in memory by adding a ‘1’ in a memory location with an address proportional to the detection time. After many photons the histogram of the detection times, i.e. the waveform of the optical pulse, builds up in the memory. Although this principle looks complicated at the first glimpse, it is very efficient and accurate for the following reasons: The accuracy of the time measurement is not limited by the width of the detector pulse. Thus, the time resolution is much better then with the same detector used in front of an oscilloscope or another linear signal acquisition device. Furthermore, all detected photons contribute to the result of the measurement. There is no loss due to ‘gating’ as in ‘Boxcar’ devices or gated image intensified CCDs.
sensitivity of the SPC method is limited mainly by the dark count rate of
the detector. Defining the sensitivity as the intensity at which the signal
is equal to the noise of the dark signal the following equation applies:
Time resolutionThe SPC method differs from methods with analog signal processing in that the time resolution is not limited by the width of the detector impulse response. For the SPC method only the timing accuracy in the detection channel is essential. This accuracy is determined by the transit time spread of the single photon pulses in the detector and the trigger accuracy in the electronic system. The timing accuracy can be up to 10 times better than the half width of the detector impulse response. Some typical values for different detector types are given below.
AccuracyThe accuracy of the measurement is given by the standard deviation of the number of collected photons in a particular time channel. For a given number of photons N the signal-to-noise ratio is SNR = N-1/2. If the light intensity is not too high, nearly all detected photons contribute to the result. Therefore, the SPC yields a very good signal-to-noise ratio at a given intensity and measurement time. Furthermore, in the SPC method, noise due leakage currents, gain instabilities, and the stochastic gain mechanism of the detector does not appear in the result. This yields an additional SNR improvement compared to analog signal processing methods.
The TCSPC method is often thought to suffer from slow recording speed and long measurement times. This bad reputation comes from traditional TCSPC devices built up from nuclear instrumentation modules which had a maximum count rate of some 104 photons per second. State-of-the-art TCSPC devices from Becker & Hickl achieve count rates of some 106 photons per seconds. Thus, 1000 photons can be collected in less than 1 ms, and the devices can be used for such high speed applications as the detection of single molecules flowing through a capillary, for fast image scanning, for the investigation of unstable samples or simply as optical oscilloscopes.
Multichannel and Multidetector Capability
Becker & Hickl has introduced multichannel and multidetector capabilities in their TCSPC modules. In the device memory space is provided for several waveforms, and the destination of each individual photon is controlled by an external signal. In conjunction with a fast scanning device, time resolved images are obtained with up to 128 x 128 pixels containing a complete waveform each. Furthermore, several detectors can be used with one TCSPC module. This technique makes use of the fact that the simultaneous detection of several photons in different detectors is very unlikely. Thus, the output pulses of several detectors are combined and an external ‘Routing’ device determines in which detector a particular photon was detected. This information is used to route the photons into different memory blocks containing the waveforms for the individual detectors.
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